{"id":39,"date":"2012-06-30T19:23:00","date_gmt":"2012-06-30T11:23:00","guid":{"rendered":"http:\/\/www.pufengdu.org\/srv\/blog\/?p=39"},"modified":"2014-11-08T19:34:14","modified_gmt":"2014-11-08T11:34:14","slug":"%e8%ae%a1%e7%ae%97fastafastq%e6%96%87%e4%bb%b6%e4%b8%ad%e5%ba%8f%e5%88%97%e7%9a%84gc-content","status":"publish","type":"post","link":"https:\/\/www.pufengdu.org\/blog\/?p=39","title":{"rendered":"\u8ba1\u7b97FASTA\/FASTQ\u6587\u4ef6\u4e2d\u5e8f\u5217\u7684GC Content"},"content":{"rendered":"<p>\u5728\u5904\u7406\u751f\u7269\u5e8f\u5217\u6570\u636e\u7684\u8fc7\u7a0b\u4e2d\uff0c\u6709\u4e9b\u65f6\u5019\u9700\u8981\u77e5\u9053\u67d0\u4e2a\u6570\u636e\u96c6\u7684GC Content\uff0c\u5982\u679c\u624b\u8fb9\u6709\u4e13\u95e8\u7684\u8f6f\u4ef6\u6700\u597d\uff0c\u5982\u679c\u6ca1\u6709\uff0c\u5176\u5b9e\u5927\u53ef\u4ee5\u7528\u7b80\u5355\u7684Linux\u547d\u4ee4\u884c\u5de5\u5177\u6765\u5927\u81f4\u7684\u8fdb\u884c\u8ba1\u7b97\u3002<\/p>\n<p>1. \u8ba1\u7b97FASTA\u6587\u4ef6\u4e2d\u7684\u603b\u7b26\u53f7\u6570\u91cf<\/p>\n<pre>\r\ncat test.fas | grep -v \"^&gt;\" | tr -d \"rn\"| wc -m\r\n<\/pre>\n<p>2. \u8ba1\u7b97FASTA\u6587\u4ef6\u4e2d\u7684\u603bGC\u6570\u91cf<\/p>\n<pre>\r\ncat test.fas | grep -v \"^&gt;\" | tr -d \"rnATNatn\" | wc -m\r\n<\/pre>\n<p>3. \u8ba1\u7b97FASTQ\u6587\u4ef6\u4e2d\u7684\u603b\u7b26\u53f7\u6570\u91cf<\/p>\n<pre>\r\ncat test.fq | awk 'BEGIN{a =0} {a++; if (a % 4 == 2) print $0}' | tr -d \"rn\" | wc -m\r\n<\/pre>\n<p>4. \u8ba1\u7b97FASTQ\u6587\u4ef6\u4e2d\u7684\u603bGC\u6570\u91cf<\/p>\n<pre>\r\ncat test.fq | awk 'BEGIN{a =0} {a++; if (a % 4 == 2) print $0}' | tr -d \"rnATNatn\" | wc -m\r\n<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>\u5728\u5904\u7406\u751f\u7269\u5e8f\u5217\u6570\u636e\u7684\u8fc7\u7a0b\u4e2d\uff0c\u6709\u4e9b\u65f6\u5019\u9700\u8981\u77e5\u9053\u67d0\u4e2a\u6570\u636e\u96c6\u7684GC Content\uff0c\u5982 &hellip; <a href=\"https:\/\/www.pufengdu.org\/blog\/?p=39\">\u7ee7\u7eed\u9605\u8bfb <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=\/wp\/v2\/posts\/39"}],"collection":[{"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=39"}],"version-history":[{"count":1,"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=\/wp\/v2\/posts\/39\/revisions"}],"predecessor-version":[{"id":195,"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=\/wp\/v2\/posts\/39\/revisions\/195"}],"wp:attachment":[{"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=39"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=39"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pufengdu.org\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=39"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}